Inoue Hirofumi, Harada Takuya, Eto Seiji, Nakashima Ken, Ibusuki Takuya, Kosha Keiko, Date Yuuko, Sanematsu Emiko, Shinohara Yoshitake, Takahashi Kojiro, Yoshimura Reiji, Nakamura Jun, Kojima Eijiro, Tsuruta Yasuto
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima, 729-0292, Japan.
Biomed Chromatogr. 2013 Jun;27(6):688-90. doi: 10.1002/bmc.2859. Epub 2013 Jan 14.
The therapeutic drug monitoring of paroxetine could be used to optimize the pharmacological treatment of depressed patients. A simple and sensitive high-performance liquid chromatography procedure was developed for the determination of paroxetine in serum. After simple pretreatment of serum (50 μL) with acetonitrile and o-phthalaldehyde, paroxetine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70°C for 20 min in borate buffer (0.1 mol/L, pH 8.0) to produce a fluorescent product. The derivative was separated on a reversed-phase column at 40°C for stepwise elution with (A) acetic acid (10 mmol/L) and (B) acetonitrile. The flow rate was 1.0 mL/min. The fluorescence intensity was monitored at excitation and emission wavelengths of 320 and 400 nm, respectively. The within-day and day-to-day relative standard deviations were 3.0-3.4 and 2.7-8.3%, respectively. The detection limit of paroxetine was 8.3 fmol at a signal-to-noise ratio of 3. As the proposed method that only requires a small quantity of serum (50 μL) is simple, sensitive and reproducible, it would be useful for clinical and biochemical research as well as drug monitoring.
帕罗西汀的治疗药物监测可用于优化抑郁症患者的药物治疗。开发了一种简单灵敏的高效液相色谱法用于测定血清中的帕罗西汀。血清（50 μL）经乙腈和邻苯二甲醛简单预处理后，帕罗西汀在硼酸盐缓冲液（0.1 mol/L，pH 8.0）中于70°C用4-(5,6-二甲氧基-2-酞酰亚胺基)-2-甲氧基苯磺酰氯衍生化20分钟，生成荧光产物。衍生物在40°C的反相柱上分离，用（A）乙酸（10 mmol/L）和（B）乙腈进行梯度洗脱。流速为1.0 mL/min。分别在激发波长320 nm和发射波长400 nm处监测荧光强度。日内和日间相对标准偏差分别为3.0 - 3.4%和2.7 - 8.3%。帕罗西汀的检测限在信噪比为3时为8.3 fmol。由于所提出的方法仅需少量血清（50 μL），简单、灵敏且可重复，因此对临床和生化研究以及药物监测将很有用。
