Department of Anesthesiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Rd., Hangzhou 310003, China.
Cell Biol Int. 2013 Mar;37(3):227-32. doi: 10.1002/cbin.10034. Epub 2013 Jan 14.
Propofol is one of the extensively commonly used intravenous anaesthetic agents. The effects of Propofol on hepatocellular carcinoma (HCC) growth inhibition and apoptosis have been examined. The techniques used were the MTT assay, flow cytometry, real-time PCR to assess miR-199a expression, as also caspase-8 and caspase-9 activity in HepG2 cells treated with Propofol. Finally, we evaluated the effect of miR-199a on Propofol-induced anti-tumour activity using anti-miR-199a. Propofol efficiently inhibited the growth of HCC cells, but was less toxic to normal hepatic cells. It induced apoptosis and increased expression of miR-199a. Activation of caspase-8 and caspase-9 suggested that both extrinsic and intrinsic pathways are involved in Propofol-induced apoptosis. Anti-miR-199a reversed the effect of Propofol on apoptosis and activation of caspase-8 and caspase-9 in HepG2 cells. Propofol can effectively induce apoptosis of HCC cells and modulation of miR-199a possibly contributes to the anti-tumour action of Propofol. Hence, Propofol might be an effective drug for HCC.
异丙酚是最广泛应用的静脉麻醉剂之一。本研究旨在探究异丙酚对肝癌（HCC）生长抑制和凋亡的影响。方法：用 MTT 法、流式细胞术、实时 PCR 检测 HepG2 细胞中转录因子 NF-κB 活性和 miR-199a 的表达，用 caspase-8 和 caspase-9 活性检测试剂盒检测 caspase-8 和 caspase-9 的活性。最后，我们用抗 miR-199a 研究 miR-199a 在异丙酚诱导的抗 HCC 活性中的作用。结果：异丙酚能有效抑制 HCC 细胞的生长，对正常肝细胞的毒性较小。它能诱导 HepG2 细胞凋亡，增加 miR-199a 的表达。caspase-8 和 caspase-9 的激活表明，外源性和内源性途径均参与了异丙酚诱导的凋亡。抗 miR-199a 逆转了 HepG2 细胞中转录因子 NF-κB 活性和 caspase-8、caspase-9 的激活。异丙酚能有效诱导 HCC 细胞凋亡，miR-199a 的调节可能有助于异丙酚的抗肿瘤作用。因此，异丙酚可能是治疗 HCC 的有效药物。
