Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1821-6. doi: 10.1073/pnas.1221306110. Epub 2013 Jan 14.
The proportion of CD4 T cells with phenotypic and functional properties of naïve cells out of total CD4 T cells is similar in the lung parenchyma and lymph nodes. On treatment with a sphingosine-1-phosphate agonist, the frequency of these cells falls precipitously, but with a delay of ∼14 h compared with blood CD4 T cells; neither anti-CD62L nor pertussis toxin prevents entry of naïve CD4 T cells into the lung. Based on treatment with anti-CD62L and the use of CCR7(-/-) cells, lung naïve CD4 T cells appear to migrate to the mediastinal lymph nodes along a CD62L-independent, CCR7-dependent pathway. Cells that have entered the node in this manner are competent to respond to antigen. Thus, a portion (approximately one-half) of naïve CD4 T cells appears to enter the mediastinal lymph nodes through a blood-to-lung-to-lymph node route.
在肺实质和淋巴结中，总 CD4 T 细胞中具有幼稚细胞表型和功能特性的 CD4 T 细胞的比例相似。用鞘氨醇-1-磷酸激动剂治疗后，这些细胞的频率急剧下降，但与血液 CD4 T 细胞相比延迟了约 14 小时；抗 CD62L 或百日咳毒素都不能阻止幼稚 CD4 T 细胞进入肺部。基于用抗 CD62L 处理和使用 CCR7(-/-)细胞，肺脏中的幼稚 CD4 T 细胞似乎沿着一种不依赖 CD62L、依赖 CCR7 的途径迁移到纵隔淋巴结。以这种方式进入淋巴结的细胞有能力对抗原作出反应。因此，一部分（大约一半）幼稚 CD4 T 细胞似乎通过血液-肺-淋巴结途径进入纵隔淋巴结。
