Krishnan Prasad, Kleiner-Hancock Heather
Department of Pharmacology, Toxicology & Neuroscience, Louisiana State University, Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA ; Department of Veterinary and Biomedical Sciences, Center for Molecular Toxicology and Carcinogenesis, Pennsylvania State University, University Park, PA 16802, USA.
Int J Breast Cancer. 2012;2012:502092. doi: 10.1155/2012/502092. Epub 2012 Dec 18.
Auraptene is being investigated for its chemopreventive effects in many models of cancer including skin, colon, prostate, and breast. Many mechanisms of action including anti-inflammatory, antiproliferative, and antiapoptotic effects are being suggested for the chemopreventive properties of auraptene. We have previously shown in the N-methylnitrosourea induced mammary carcinogenesis model that dietary auraptene (500 ppm) significantly delayed tumor latency. The delay in time to tumor corresponded with a significant reduction in cyclin D1 protein expression in the tumors. Since cyclin D1 is a major regulator of cell cycle, we further studied the effects of auraptene on cell cycle and the genes related to cell cycle in MCF-7 cells. Here we show that auraptene significantly inhibited IGF-1 stimulated S phase of cell cycle in MCF-7 cells and significantly changed the transcription of many genes involved in cell cycle.
金柑黄酮正在多种癌症模型（包括皮肤癌、结肠癌、前列腺癌和乳腺癌）中接受化学预防作用的研究。对于金柑黄酮的化学预防特性，人们提出了多种作用机制，包括抗炎、抗增殖和抗凋亡作用。我们之前在N-甲基亚硝基脲诱导的乳腺癌发生模型中表明，饮食中的金柑黄酮（500 ppm）显著延迟了肿瘤潜伏期。肿瘤发生时间的延迟与肿瘤中细胞周期蛋白D1蛋白表达的显著降低相对应。由于细胞周期蛋白D1是细胞周期的主要调节因子，我们进一步研究了金柑黄酮对MCF-7细胞中细胞周期以及与细胞周期相关基因的影响。在此我们表明，金柑黄酮显著抑制了MCF-7细胞中IGF-1刺激的细胞周期S期，并显著改变了许多参与细胞周期的基因的转录。
