Andolfatto Carolina, da Silva Guilherme Ferreira, Cornélio Ana Livia Gomes, Guerreiro-Tanomaru Juliane Maria, Tanomaru-Filho Mario, Faria Gisele, Bonetti-Filho Idomeo, Cerri Paulo Sérgio
Department of Restorative Dentistry, Araraquara Dental School, Universidade Estadual Paulista (UNESP), 14801-903 Araraquara, SP, Brazil.
ISRN Dent. 2012;2012:904963. doi: 10.5402/2012/904963. Epub 2012 Dec 18.
Objective. The aim of this study was to evaluate the rat subcutaneous tissue reaction to calcium hydroxide-based intracanal medicaments, UltraCal XS (calcium hydroxide, barium sulphate, aqueous matrix), Hydropast (calcium hydroxide, barium sulphate, and propyleneglycol), and Calen (Calcium hydroxide, zinc oxide, colophony, and polyethyleneglycol), used as a control. Methods. Forty-eight rats (Rattus Norvegicus Holtzman) were distributed in three groups: Calen, UltraCal XS, and Hydropast. Polyethylene tubes filled with one of the medicaments were implanted in the dorsal subcutaneous. After 7 and 30 days, the implants were removed and the specimens were fixed and embedded in paraffin. Morphological and quantitative analyses were carried out in the HE-stained sections. The numerical density of inflammatory cells in the capsule was evaluated and statistical analyses were performed (P ≤ 0.05). Results. At 7 days, all materials induced an inflammatory reaction in the subcutaneous tissue adjacent to the implants. In all groups, a significant reduction in the number of inflammatory cells and giant cells was verified in the period of 30 days. Conclusion. These results indicate that the calcium hydroxide-based medicaments evaluated present biocompatibility similar to Calen.
目的。本研究旨在评估大鼠皮下组织对基于氢氧化钙的根管内药物UltraCal XS(氢氧化钙、硫酸钡、水性基质)、Hydropast(氢氧化钙、硫酸钡和丙二醇)以及用作对照的Calen(氢氧化钙、氧化锌、松香和聚乙二醇)的反应。方法。将48只大鼠(挪威大鼠霍尔兹曼品系)分为三组:Calen组、UltraCal XS组和Hydropast组。将填充有一种药物的聚乙烯管植入背部皮下。7天和30天后,取出植入物,将标本固定并石蜡包埋。对苏木精-伊红染色切片进行形态学和定量分析。评估包膜中炎性细胞的数值密度并进行统计分析(P≤0.05)。结果。7天时,所有材料均在植入物邻近的皮下组织中引发炎症反应。在所有组中,30天时炎性细胞和巨细胞数量均显著减少。结论。这些结果表明,所评估的基于氢氧化钙的药物具有与Calen相似的生物相容性。