在非洲爪蟾卵母细胞的线粒体基质中，三磷酸腺苷（ATP）抑制作用在调节烟酰胺腺嘌呤二核苷酸（磷酸）苹果酸酶活性时与激活阳离子相互竞争。
ATP inhibition competes with activating cations in modulating the NAD(P)(+)-malic enzyme activity in the mitochondrial matrix of Xenopus laevis oocytes.
作者信息
Petrucci D, Cesare P
机构信息
Department of Cell Biology and Physiology, University of L'Aquila, Italy.
出版信息
Int J Biochem. 1990;22(2):137-41. doi: 10.1016/0020-711x(90)90175-3.
PMID:2332098
Abstract
- ATP inhibits NAD(P)(+)-dependent malic enzyme activity by competing with the essential activators Mn2+ and Mg2+. 2. The kinetics fit an equation of co-operative kind with Ki of 26 microM and KA of 11.3 microM for ATP/Mn2+ competition; with Ki of 1.1 mM and KA of 0.96 mM for ATP/Mg2+ competition. 3. In the absence of the inhibitor, the co-operativity index increases from 1.77 to greater than 4 in the presence of ATP, in the case of ATP/Mn2+ competition, while it increases from 1.88 to greater than 9 for ATP/Mg2+ competition.
摘要
- ATP通过与必需激活剂Mn2+和Mg2+竞争来抑制NAD(P)(+)依赖性苹果酸酶活性。2. 动力学符合协同类型方程，ATP/Mn2+竞争时Ki为26 microM，KA为11.3 microM；ATP/Mg2+竞争时Ki为1.1 mM，KA为0.96 mM。3. 在不存在抑制剂的情况下，对于ATP/Mn2+竞争，协同指数在有ATP存在时从1.77增加到大于4，而对于ATP/Mg2+竞争，协同指数从1.88增加到大于9。
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