兔碱性磷酸酶抗原性和糖链异质性的发育变化

Developmental changes in the antigenicity and sugar-chain heterogeneity of rabbit alkaline phosphatases.

Arai Y, Koyama I, Hirota N, Sakai T, Sakagishi Y, Komoda T

Department of Biochemistry, Saitama Medical School, Japan.

Int J Biochem. 1990;22(2):143-52. doi: 10.1016/0020-711x(90)90176-4.

Abstract

Rabbit alkaline phosphatases (APs) clearly fused with the anti-human AP antibodies. In particular, fetal liver and kidney APs reacted slightly less with the anti-intestinal AP antibody as did adult enzymes, suggesting that intestinal AP-like isozyme is expressed at earlier stages of gestation in rabbit liver and kidney. 2. Immunohistochemical data indicated that intestinal AP-like isozyme in the kidney was mainly localized in the distal convoluted tubules and slightly in the proximal straight tubules, whereas liver/bone/kidney AP-like enzyme was found more in the glomeruli and interstitial capillary walls as a major component. 3. The sugar-chain heterogeneity of adult and fetal rabbit APs displayed organ-specificity as did of rat and human APs. Moreover, in fetal development, the expression of high-mannose type or hybrid type sugar chains precedes the expression of complex type sugar chains in fetal development.

摘要

兔碱性磷酸酶（APs）与抗人AP抗体明显融合。特别是，胎儿肝脏和肾脏的APs与抗肠道AP抗体的反应比成年酶稍弱，这表明肠道AP样同工酶在兔肝脏和肾脏妊娠的早期阶段就有表达。2. 免疫组织化学数据表明，肾脏中的肠道AP样同工酶主要定位于远曲小管，在近端直小管中含量较少，而肝/骨/肾AP样酶作为主要成分更多地存在于肾小球和间质毛细血管壁中。3. 成年和胎儿兔APs的糖链异质性与大鼠和人APs一样表现出器官特异性。此外，在胎儿发育过程中，高甘露糖型或杂合型糖链的表达先于复杂型糖链在胎儿发育中的表达。