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嗜盐栖嗜盐菌趋化缺陷突变体的特性分析

Characterization of Halobacterium halobium mutants defective in taxis.

作者信息

Sundberg S A, Alam M, Lebert M, Spudich J L, Oesterhelt D, Hazelbauer G L

机构信息

Cardiovascular Research Institute, University of California, San Francisco 94143.

出版信息

J Bacteriol. 1990 May;172(5):2328-35. doi: 10.1128/jb.172.5.2328-2335.1990.

Abstract

Mutant derivatives of Halobacterium halobium previously isolated by using a procedure that selected for defective phototactic response to white light were examined for an array of phenotypic characteristics related to phototaxis and chemotaxis. The properties tested were unstimulated swimming behavior, behaviorial responses to temporal gradients of light and spatial gradients of chemoattractants, content of photoreceptor pigments, methylation of methyl-accepting taxis proteins, and transient increases in rate of release of volatile methyl groups induced by tactic stimulation. Several distinct phenotypes were identified, corresponding to a mutant missing photoreceptors, a mutant defective in the methyltransferase, a mutant altered in control of the methylesterase, and mutants apparently defective in intracellular signaling. All except the photoreceptor mutant were defective in both chemotaxis and phototaxis.

摘要

利用一种选择对白光的趋光反应有缺陷的程序先前分离出的嗜盐嗜盐菌突变衍生物,针对一系列与趋光性和趋化性相关的表型特征进行了检测。所测试的特性包括未受刺激的游动行为、对光的时间梯度和化学引诱剂的空间梯度的行为反应、光受体色素的含量、甲基接受趋化蛋白的甲基化以及趋化刺激诱导的挥发性甲基基团释放速率的瞬时增加。鉴定出了几种不同的表型,分别对应于缺失光受体的突变体、甲基转移酶有缺陷的突变体、甲基酯酶控制发生改变的突变体以及细胞内信号传导明显有缺陷的突变体。除了光受体突变体之外,所有突变体在趋化性和趋光性方面均有缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0854/208866/fd9560f8dd2c/jbacter00119-0145-a.jpg

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