野油菜黄单胞菌野油菜致病变种中参与胞外多糖产生的35.3千碱基DNA区域的克隆与分析
Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
作者信息
Hötte B, Rath-Arnold I, Pühler A, Simon R
机构信息
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Federal Republic of Germany.
出版信息
J Bacteriol. 1990 May;172(5):2804-7. doi: 10.1128/jb.172.5.2804-2807.1990.
Abstract
Cosmid clones able to restore exopolysaccharide production in possibly insertion sequence element-induced surface mutants of Xanthomonas campestris pv. campestris were isolated. By fragment-specific Tn5-lac mutagenesis of one of the cosmids, pXCB1002, a new DNA region which is involved in exopolysaccharide biosynthesis and which is organized into at least 12 complementation groups was identified.
摘要
分离出了能够恢复野油菜黄单胞菌野油菜致病变种可能由插入序列元件诱导的表面突变体中外多糖产生的黏粒克隆。通过对其中一个黏粒pXCB1002进行片段特异性Tn5-lac诱变，鉴定出一个参与外多糖生物合成且至少由12个互补组组成的新DNA区域。
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