埃兹蛋白沉默对胰腺癌细胞系Panc-1的影响

[Effects of ezrin silencing on pancreatic cancer cell line Panc-1].

作者信息

Meng Yun-xiao, Yu Shuang-ni, Lu Zhao-hui, Chen Jie

机构信息

Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2012 Dec;41(12):833-6. doi: 10.3760/cma.j.issn.0529-5807.2012.12.009.

DOI:10.3760/cma.j.issn.0529-5807.2012.12.009

PMID:23324233

Abstract

OBJECTIVE

To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.

METHODS

Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid. The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis, respectively. Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy. Colony formation assay was used to determine the cell anchor-independent growth ability in vitro. Trans-filter migration and invasion assays were performed with 8 µm pore inserts in a 24-well BioCoat chamber with/without Matrigel.

RESULTS

Ezrin silencing decreased cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion, but had no effects on cell proliferation in vitro and cell cycle, in pancreatic cancer cell line Panc-1.

CONCLUSION

Ezrin expression affects the cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion in pancreatic cancer cell line Panc-1.

摘要

目的

探讨埃兹蛋白沉默对胰腺癌细胞系Panc-1的影响。

方法

用埃兹蛋白沉默质粒转染胰腺癌细胞系Panc-1。分别通过CCK-8法和流式细胞术分析测定细胞增殖和细胞周期状态。通过扫描电子显微镜观察细胞膜突起/微绒毛的形成。采用集落形成试验测定细胞体外非锚定依赖性生长能力。使用8μm孔径的Transwell小室在含/不含基质胶的24孔BioCoat培养板中进行跨膜迁移和侵袭试验。