Shinoda Y, Shouji A, Yamane T, Suzuki A, Ashida T, Kihara H, Ohno M
Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Aichi.
J Biochem. 1990 Jan;107(1):84-6. doi: 10.1093/oxfordjournals.jbchem.a123017.
Crystallization and a preliminary crystallographic study of Trimeresurus flavoviridis (habu snake) phospholipase A2 (PLA2) were carried out. Although crystals were obtained from various solutions, crystals suitable for X-ray analysis could be obtained from polyethylene glycol solutions only when a repeated seeding technique was applied starting from twinned crystals. The crystal is monoclinic with space group P21, with a = 44.1, b = 55.7, c = 48.8 A, and beta = 92.4 degrees. An asymmetric unit contains a dimer consisting of two identical subunits made of 122 amino acids. The crystal reflects X-rays beyond 2.5 A. A Pt derivative gave a good isomorphous crystal.
