Institut de Topologie et de Dynamique des Systèmes, UMR 7086 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, 75205 Paris Cedex, France.
Bioorg Med Chem Lett. 2013 Feb 15;23(4):955-8. doi: 10.1016/j.bmcl.2012.12.057. Epub 2012 Dec 22.
Oxime bond formation has been applied to the preparation of oligonucleotides labeled with electrochemical ferrocene and viologen labels. Aminooxy functionalized ferrocene and viologen derivatives were prepared by a straightforward route and efficiently conjugated with aldehyde containing oligonucleotides either at 3' or 5' end. Both labels were found to not disturb the recognition properties of the oligonucleotide. The versatility of the method was further demonstrated by preparing bi-functionalized conjugates with a disulfide at 3' end and an electrochemical label at 5' end.
肟键形成已被应用于制备用电化学的二茂铁和紫精标记的寡核苷酸。通过一种直接的路线制备了氨基氧功能化的二茂铁和紫精衍生物，并有效地与含有醛的寡核苷酸在 3' 或 5' 端连接。发现这两个标记都不会干扰寡核苷酸的识别特性。该方法的多功能性还通过在 3' 端制备带有二硫键和在 5' 端制备电化学标记的双功能化缀合物得到了进一步证明。
