由RNA聚合酶II和从肝脏中纯化的转录因子启动转录。转录因子tau和epsilon在初始复合物形成中的协同作用。
Transcription initiated by RNA polymerase II and purified transcription factors from liver. Cooperative action of transcription factors tau and epsilon in initial complex formation.
作者信息
Conaway J W, Reines D, Conaway R C
机构信息
Department of Chemistry, University of Texas, Austin 78712-1096.
出版信息
J Biol Chem. 1990 May 5;265(13):7552-8.
Synthesis of accurately initiated transcripts has been reconstituted with RNA polymerase II and a set of five transcription factors purified from rat liver. In addition to three previously identified factors alpha, beta gamma, and delta (Conaway, R. C., and Conaway, J. W. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 7356-7360), transcription in the reconstituted liver system requires two novel factors designated tau and epsilon. These five transcription factors comprise two functional classes: (i) promoter recognition factors (tau and epsilon), which interact with template DNA to facilitate formation of a stable initial complex that is subsequently recognized and bound by RNA polymerase II, and (ii) RNA chain initiation factors (alpha, beta gamma, and delta), which do not participate in formation of the initial complex, but which are essential for transcription initiation.
已利用从大鼠肝脏中纯化的RNA聚合酶II和一组五种转录因子重建了精确起始转录本的合成。除了三种先前鉴定出的因子α、βγ和δ（康纳韦，R.C.，和康纳韦，J.W.（1989年）美国国家科学院院刊86，7356 - 7360）之外，重建的肝脏系统中的转录还需要两种新的因子，分别命名为τ和ε。这五种转录因子包括两个功能类别：（i）启动子识别因子（τ和ε），它们与模板DNA相互作用以促进稳定初始复合物的形成，随后该复合物被RNA聚合酶II识别并结合；（ii）RNA链起始因子（α、βγ和δ），它们不参与初始复合物的形成，但对于转录起始至关重要。
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