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聚合酶II和聚合酶III转录中的TATA结合蛋白及相关因子

TATA-binding protein and associated factors in polymerase II and polymerase III transcription.

作者信息

Meyers R E, Sharp P A

机构信息

Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, 02139.

出版信息

Mol Cell Biol. 1993 Dec;13(12):7953-60. doi: 10.1128/mcb.13.12.7953-7960.1993.

DOI:10.1128/mcb.13.12.7953-7960.1993
PMID:8247010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC364867/
Abstract

Transcription by RNA polymerase I (pol I), pol II, and pol III requires the TATA-binding protein (TBP). This protein functions in association with distinct TBP-associated factors (TAFs) which may specify the nature of the polymerase selected for initiation at a promoter site. In the pol III transcription system, the TBP-TAF complex is a component of the TFIIIB factor. This factor has been resolved into a TBP-TAF complex and another component, both of which are required for reconstitution of transcription by pol III. Neither the TBP-TAF complexes B-TFIID and D-TFIID, which were previously characterized as active for pol II transcription, nor TBP alone can complement pol III transcription reactions that are dependent upon the TBP-TAF subcomponent of TFIIIB. Surprisingly, the TBP-TAF subcomponent of TFIIIB is active in reconstitution of pol II transcription.

摘要

RNA聚合酶I（pol I）、聚合酶II和聚合酶III的转录需要TATA结合蛋白（TBP）。该蛋白与不同的TBP相关因子（TAFs）共同发挥作用，这些因子可能决定在启动子位点选择用于起始转录的聚合酶的性质。在聚合酶III转录系统中，TBP-TAF复合物是TFIIIB因子的一个组成部分。该因子已被解析为一个TBP-TAF复合物和另一个组分，这两者都是聚合酶III进行转录重建所必需的。之前被鉴定为对聚合酶II转录有活性的TBP-TAF复合物B-TFIID和D-TFIID，以及单独的TBP，都不能补充依赖于TFIIIB的TBP-TAF亚组分的聚合酶III转录反应。令人惊讶的是，TFIIIB的TBP-TAF亚组分在聚合酶II转录重建中具有活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/8ded744554a7/molcellb00024-0769-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/990285217edf/molcellb00024-0767-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/990285217edf/molcellb00024-0767-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/0ccdc37ddcf5/molcellb00024-0768-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/736986a47ee7/molcellb00024-0768-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/3ac2eaed52be/molcellb00024-0769-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d9d/364867/8ded744554a7/molcellb00024-0769-b.jpg

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DNA topology and a minimal set of basal factors for transcription by RNA polymerase II.DNA拓扑结构与RNA聚合酶II转录所需的一组最小基本因子
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