Department of Family Medicine, China Medical University Hospital, School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan.
Dis Markers. 2013;34(3):187-97. doi: 10.3233/DMA-120961.
Previous studies inferring that the NOS3 gene was associated with the pathogenesis of metabolic syndrome (MetS) had inconsistent findings. We investigated the role of three NOS3 polymorphisms (T-786C, intron 4b/a, and G894T) in the risk of MetS using a hospital-based case-control study.
We recruited 339 MetS cases and 783 non-MetS controls at a central Taiwanese hospital. Information on sociodemographic and lifestyle factors was obtained using a self-administered questionnaire. Genotypes of NOS3 polymorphisms were compared between cases and controls. Effects of interactions between gene polymorphisms and smoking and between gene polymorphisms and drinking on the risk of MetS were also determined.
The T-786C TC+CC genotype was significantly associated with a decreased risk of MetS (odds ratio (OR), 0.63; 95% confidence interval (CI), 0.43-0.91), compared to the T-786C TT genotype, according to a logistic regression analysis. This beneficial effect was much greater for those who had ever smoked cigarettes (OR, 0.47; 95% CI, 0.26-0.87) or those who had not consumed alcohol (OR, 0.45; 95% CI, 0.26-0.77). In addition, the intron 4b/a variant genotype was marginally associated with a reduced risk of MetS (OR, 0.68; 95% CI, 0.47-1.00), compared to the intron 4b/a bb genotype, particularly for never alcohol consumers (OR, 0.56; 95% CI, 0.33-0.95). In the haplotype analysis, there was a 53% decrease in the MetS risk among C4bG haplotype carriers (OR, 0.47; 95% CI, 0.25-0.90), compared to those with the most common T4bG haplotype.
Our results suggest that the NOS3 T-786C and intron 4b/a polymorphisms may contribute to the risk of MetS. Further studies are needed to confirm the findings.
先前的研究推断，NOS3 基因与代谢综合征（MetS）的发病机制有关，但研究结果并不一致。我们采用基于医院的病例对照研究，调查了三个 NOS3 基因多态性（T-786C、内含子 4b/a 和 G894T）在 MetS 发病风险中的作用。
我们在台湾中部的一家医院招募了 339 例 MetS 病例和 783 名非 MetS 对照。采用自填式问卷收集了社会人口统计学和生活方式因素的信息。比较了病例和对照之间 NOS3 基因多态性的基因型。还确定了基因多态性与吸烟和饮酒之间的相互作用以及基因多态性与饮酒之间的相互作用对 MetS 风险的影响。
与 T-786C TT 基因型相比，根据逻辑回归分析，T-786C TC+CC 基因型与 MetS 风险降低显著相关（比值比（OR），0.63；95%置信区间（CI），0.43-0.91）。对于曾经吸烟（OR，0.47；95%CI，0.26-0.87）或不饮酒（OR，0.45；95%CI，0.26-0.77）的人，这种有益作用更大。此外，与 intron 4b/a bb 基因型相比，内含子 4b/a 变体基因型与 MetS 风险降低呈边缘相关（OR，0.68；95%CI，0.47-1.00），尤其是从不饮酒的人（OR，0.56；95%CI，0.33-0.95）。在单倍型分析中，与最常见的 T4bG 单倍型相比，C4bG 单倍型携带者 MetS 风险降低了 53%（OR，0.47；95%CI，0.25-0.90）。
我们的结果表明，NOS3 T-786C 和内含子 4b/a 多态性可能有助于 MetS 的发病风险。需要进一步的研究来证实这些发现。
