小鼠模型中HPV感染的光学成像

Optical imaging of HPV infection in a murine model.

作者信息

Kines Rhonda C, Kobayashi Hisataka, Choyke Peter L, Bernardo Marcelino L

机构信息

Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2013;961:141-50. doi: 10.1007/978-1-62703-227-8_7.

DOI:10.1007/978-1-62703-227-8_7

PMID:23325640

Abstract

The development of animal models of HPV infection has given investigators a new set of tools to expand basic knowledge of the early events of infection in vivo. The use of HPV pseudovirions, in which the viral genome has been replaced with a reporter pseudogenome, in combination with advanced imaging techniques has facilitated and simplified studies using these models. Herein we provide details for a murine model of cervicovaginal HPV infection in conjunction with several methods for imaging and quantitating the transduced genes, both ex vivo and in vivo.

摘要

人乳头瘤病毒（HPV）感染动物模型的发展为研究人员提供了一套新工具，以拓展对体内感染早期事件的基础知识。使用其中病毒基因组已被报告假基因组取代的HPV假病毒颗粒，结合先进的成像技术，促进并简化了使用这些模型的研究。在此，我们提供了宫颈阴道HPV感染小鼠模型的详细信息，以及几种用于在体外和体内对转导基因进行成像和定量的方法。