Pasmooij Anna M G, Jonkman Marcel F
Department of Dermatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Methods Mol Biol. 2013;961:165-77. doi: 10.1007/978-1-62703-227-8_9.
Laser Dissection Microscopy (LDM) is a valuable technique to identify the genetic event that underlies cutaneous mosaicism. Isolation of a selected cell population is key to the detection of a somatic mutation. Advantages of the LDM technique are that stored skin biopsies can be analyzed without the necessity of culturing cells. Here, we describe the preparation of skin sections and cells for LDM, followed by dissection, and subsequent DNA and RNA analysis in order to detect the underlying somatic mutation.
激光显微切割术（LDM）是一种用于识别皮肤镶嵌现象潜在遗传事件的重要技术。分离选定的细胞群体是检测体细胞突变的关键。LDM技术的优点是可以对储存的皮肤活检样本进行分析，而无需培养细胞。在此，我们描述了用于LDM的皮肤切片和细胞的制备方法，随后进行切割，以及后续的DNA和RNA分析，以检测潜在的体细胞突变。
