Ishii Ken, Amagai Masayuki
Department of Dermatology, Toho University, School of Medicine, Tokyo, Japan.
Methods Mol Biol. 2013;961:219-25. doi: 10.1007/978-1-62703-227-8_13.
Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.
天疱疮患者体内存在循环抗桥粒芯糖蛋白（Dsg）1和/或Dsg3自身抗体，这些抗体可导致皮肤和黏膜形成水疱。我们在此描述两种检测水疱形成中自身抗体致病强度的方法：一种是使用原代人表皮角质形成细胞的体外解离试验，以评估抗Dsg3自身抗体的致病性；另一种替代方法是将抗Dsg3和Dsg1自身抗体注射到器官培养的人皮肤标本中。
