Carretero Marta, Guerrero-Aspizua Sara, Del Río Marcela
Epithelial Biomedicine Division, Basic Research Department, Centro de Investigaciones Energéticas, Medioambientales, y Tecnológicas, Centre for Biomedical Research on Rare Diseases U714, Madrid, Spain.
Methods Mol Biol. 2013;961:305-23. doi: 10.1007/978-1-62703-227-8_20.
This protocol describes the generation of a skin humanized mouse model for psoriasis using bioengineering approaches. This method is relatively simple, highly reproducible and ensures the obtention of a large and homogenous number of engrafted animals bearing a portion of human skin with psoriatic phenotype. The technique can employ cells from skin biopsies and blood samples from non-related healthy human donors (allogeneic version), as well as skin and blood cells from psoriatic patients (autologous version). In both cases, the psoriatic phenotype was developed after intradermal administration of in vitro derived T1 lymphocytes along with Th17 recombinant cytokines, in conjunction with mild barrier disruption by tape-stripping. This skin-humanized model for psoriasis emerges as a powerful tool to study the mechanisms underlying the pathogenesis of the disease. More importantly, the feasibility of the system may allow the evaluation of different therapeutic compounds in an in vivo system, employing local and/or systemic administration.
本方案描述了使用生物工程方法生成用于银屑病的皮肤人源化小鼠模型。该方法相对简单，具有高度可重复性，并确保获得大量具有银屑病表型的人皮肤移植的同基因动物。该技术可使用来自非相关健康人类供体的皮肤活检细胞和血液样本（同种异体版本），以及银屑病患者的皮肤和血细胞（自体版本）。在这两种情况下，在皮内注射体外衍生的T1淋巴细胞以及Th17重组细胞因子后，结合胶带剥离造成的轻度屏障破坏，出现了银屑病表型。这种银屑病皮肤人源化模型成为研究该疾病发病机制的有力工具。更重要的是，该系统的可行性可能允许在体内系统中使用局部和/或全身给药来评估不同的治疗化合物。
