Fischer Michael R, John Dominik, Kautz-Neu Kordula, Schermann Anja I, Schwonberg Kirsten, von Stebut Esther
Department of Dermatology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
Methods Mol Biol. 2013;961:389-402. doi: 10.1007/978-1-62703-227-8_26.
Using cutaneous leishmaniasis of mice, the existence of so-called T helper (Th) cells type 1 and type 2 had been identified more than 20 years ago. Nowadays, it is well accepted that additional T cell populations as well as B cell-mediated immunity is required for immunity against Leishmania major. Finally, using inbred mouse strains, the relevance of genetical factors that influence anti-pathogen immunity as well as elements of the skin-immune system have been identified. This protocol describes a model for murine experimental leishmaniasis that tries to mimic natural parasite transmission by several means: (1) utilization of only infectious-stage parasites that are found in sand fly saliva, (2) intradermal inoculation, and (3) infection with only 1,000 parasites similar to the numbers inoculated by an infected sand fly.
20多年前，利用小鼠皮肤利什曼病，就已证实了所谓1型和2型辅助性T（Th）细胞的存在。如今，人们普遍认为，抗硕大利什曼原虫免疫需要额外的T细胞群体以及B细胞介导的免疫。最后，利用近交系小鼠品系，已确定了影响抗病原体免疫的遗传因素以及皮肤免疫系统各成分的相关性。本方案描述了一种小鼠实验性利什曼病模型，该模型试图通过多种方式模拟自然寄生虫传播：（1）仅使用在白蛉唾液中发现的感染期寄生虫；（2）皮内接种；（3）仅用1000个寄生虫进行感染，这一数量与受感染白蛉接种的数量相似。
