College of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China.
Pest Manag Sci. 2013 Aug;69(8):983-9. doi: 10.1002/ps.3463. Epub 2013 Jan 16.
The influence of adding trehalose to the culture medium on the efficacy of Pichia caribbica (JSU-1) in controlling post-harvest grey mould and blue mould decay of apples and the possible mechanisms involved were investigated.
The antagonistic activity of P. caribbica harvested from nutrient yeast dextrose broth (NYDB) amended with trehalose at 5 mg mL(-1) to post-harvest grey mould and blue mould decay was improved greatly compared with that without trehalose. The intracellular trehalose content of P. caribbica harvested from NYDB amended with trehalose at 5 mg mL(-1) was higher than that of P. caribbica harvested from NYDB. The population of P. caribbica could rapidly colonise and grow in apple wounds. Moreover, the application of P. caribbica cultivated in the culture medium of the trehalose supplement (5 mg mL(-1) ) induced higher polyphenoloxidase (PPO) and peroxidase (POD) activity of apples compared with that cultivated by P. caribbica in the NYDB. SEM results suggested that P. caribbica harvested from NYDB amended with trehalose at 5 mg mL(-1) showed stronger inhibition efficacy to Botrytis cinerea than P. caribbica harvested from NYDB.
The antagonistic activity of P. caribbica can be enhanced by adding trehalose to the medium.
研究了在培养基中添加海藻糖对毕赤酵母（JSU-1）控制苹果采后灰霉病和青霉病腐烂的功效的影响及其可能的作用机制。
与不添加海藻糖相比，在添加 5mg/mL 海藻糖的营养酵母葡萄糖肉汤（NYDB）中收获的毕赤酵母对采后灰霉病和青霉病腐烂的拮抗活性有了很大提高。在添加 5mg/mL 海藻糖的 NYDB 中收获的毕赤酵母的细胞内海藻糖含量高于在 NYDB 中收获的毕赤酵母。毕赤酵母能迅速在苹果伤口定殖和生长。此外，与在 NYDB 中培养的毕赤酵母相比，在添加海藻糖（5mg/mL）的培养基中培养的毕赤酵母诱导的苹果多酚氧化酶（PPO）和过氧化物酶（POD）活性更高。SEM 结果表明，与在 NYDB 中收获的毕赤酵母相比，在添加 5mg/mL 海藻糖的 NYDB 中收获的毕赤酵母对葡萄孢菌的抑制效果更强。
通过在培养基中添加海藻糖可以增强毕赤酵母的拮抗活性。
