Cojocarui Inimioara Mihaela, Cojocaru M, Sapira Violeta, Socoliuc Gabriela, Hertea Cristina, Paveliu S
"Carol Davila" University of Medicine and Pharmacy, Colentina Clinical Hospital, Department of Neurology, Bucharest, Romania.
Rom J Intern Med. 2012 Apr-Jun;50(2):155-8.
Matrix metalloproteinases (MMP) have been thought to be involved in stroke pathogenesis. MMP-9 contributes to tissue destruction. Our aim was to analyze the MMP-9 levels in blood within 24 hours of acute ischemic stroke onset to observe the role of MMP-9 in the pathogenesis of atherothrombotic stroke. In this study we investigated prospectively MMP-9 levels in serum from 106 patients (42 men and 64 women, mean age 71.52 +/- 6.32 years) with acute ischemic stroke in the middle cerebral artery area in the first 24 hours from the onset (mean duration 7.8 +/- 4.5 hours) as compared to 112 controls (48 men and 64 women, mean age 70.36 +/- 6.8 years). Serum samples were collected under sterile conditions and stored in aliquots at -70 degrees C until assay. Serum MMP-9 levels were determined by enzyme-linked immunosorbent assay (ELISA) in blood samples obtained on admission. Statistical analysis was performed by Mann-Whitney and Log-Likeliwood Ratio tests. All values reported are expressed as mean (x) +/- SD. Mean serum MMP-9 concentrations were higher in group with ischemic stroke 172 +/- 32.4 ng/mL, range 139.6-204.4 ng/mL vs. controls 57 +/- 9.6 ng/mL, range 47.4-66.6 ng/mL (95% CI, 3.17 to 14.18; p < 0.014). In conclusion, MMP-9 activity is associated with early acute ischemic stroke. The high levels of MMP-9 in acute ischemic stroke document the involvement of this enzyme in the regulation of inflammation in stroke.
基质金属蛋白酶（MMP）被认为与中风发病机制有关。MMP-9会导致组织破坏。我们的目的是分析急性缺血性中风发病后24小时内血液中的MMP-9水平，以观察MMP-9在动脉粥样硬化血栓形成性中风发病机制中的作用。在本研究中，我们前瞻性地调查了106例急性缺血性中风患者（42例男性和64例女性，平均年龄71.52±6.32岁）在发病后头24小时内（平均病程7.8±4.5小时）大脑中动脉区域血清中的MMP-9水平，并与112例对照组（48例男性和64例女性，平均年龄70.36±6.8岁）进行比较。血清样本在无菌条件下采集，并分装保存在-70℃直至检测。通过酶联免疫吸附测定（ELISA）法测定入院时采集的血液样本中的血清MMP-9水平。采用Mann-Whitney检验和对数似然比检验进行统计分析。所有报告的值均表示为平均值（x）±标准差。缺血性中风组的平均血清MMP-9浓度较高，为172±32.4 ng/mL，范围为139.6 - 204.4 ng/mL，而对照组为57±9.6 ng/mL，范围为47.4 - 66.6 ng/mL（95%CI，3.17至14.18；p < 0.014）。总之，MMP-9活性与早期急性缺血性中风有关。急性缺血性中风中MMP-9的高水平表明该酶参与了中风炎症的调节。
