Buraczynska Kinga, Kurzepa Jacek, Ksiazek Andrzej, Buraczynska Monika, Rejdak Konrad
Department of Neurology, Medical University of Lublin, Dr K. Jaczewskiego 8, 20-954, Lublin, Poland.
Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland.
Neuromolecular Med. 2015 Dec;17(4):385-90. doi: 10.1007/s12017-015-8367-5. Epub 2015 Sep 2.
Matrix metalloproteinases (MMPs), endopeptidases degrading extracellular matrix, play an important role in the pathogenesis of atherosclerosis and vascular disease. The aim of this study was to evaluate the association between the C(-1562)T functional polymorphism in the MMP-9 gene and risk of stroke. We examined 322 patients with stroke and 410 controls. In the patient group, 52 % had type 2 diabetes. All subjects were genotyped for the C(-1562)T polymorphism by polymerase chain reaction and restriction analysis. A significant increase in T allele and CT + TT genotype frequencies was observed in patients compared with controls (OR 1.73, 95 % CI 1.34-2.23 and 1.89, 95 % CI 1.39-2.56, respectively). The T allele carriers were younger at the onset of stroke (63.5 ± 11.7 years) than patients with CC genotype (71 ± 14.1 years) (p = 0.0002). The comparison between patients with T2DM and without it showed that the T allele and CT + TT genotype were more frequent in T2DM patients (OR 1.48, 95 % CI 1.03-2.12 for T allele and 1.44, 95 % CI 1.93-2.24 for CT + TT genotype). In conclusion, our findings suggest that MMP-9 C(-1562)T polymorphism is significantly associated with risk of stroke in patients with and without T2DM.
基质金属蛋白酶（MMPs）是降解细胞外基质的内肽酶，在动脉粥样硬化和血管疾病的发病机制中起重要作用。本研究的目的是评估MMP - 9基因中C（-1562）T功能多态性与中风风险之间的关联。我们检查了322例中风患者和410例对照。在患者组中，52%患有2型糖尿病。通过聚合酶链反应和限制性分析对所有受试者的C（-1562）T多态性进行基因分型。与对照组相比，患者中T等位基因和CT + TT基因型频率显著增加（OR分别为1.73，95%CI为1.34 - 2.23和1.89，95%CI为1.39 - 2.56）。T等位基因携带者中风发病时比CC基因型患者年轻（63.5±11.7岁对71±14.1岁）（p = 0.0002）。有和没有2型糖尿病患者之间的比较表明，T等位基因和CT + TT基因型在2型糖尿病患者中更常见（T等位基因的OR为1.48，95%CI为1.03 - 2.12；CT + TT基因型的OR为1.44，95%CI为1.93 - 2.24）。总之，我们的研究结果表明，MMP - 9 C（-1562）T多态性与有和没有2型糖尿病患者的中风风险显著相关。
