Thomas H M, Green I C, Wallis M, Aston R
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton.
J Endocrinol. 1990 Mar;124(3):469-74. doi: 10.1677/joe.0.1240469.
Male hypophysectomized rats treated with bovine (b)GH-monoclonal antibody complexes showed enhanced weight gain compared with animals treated with bGH alone over a 12-day treatment period. Liver microsomes prepared from animals showing enhanced weight gain exhibited increased specific binding of human (h)GH. Studies on the specificity of these binding sites showed that they were lactogenic, 125I-labelled hGH being displaced by ovine prolactin, but not by non-mammalian growth hormones. In this respect they were similar to lactogenic binding sites in the liver of pregnant rats. Monoclonal antibodies to hGH blocked binding to lactogenic receptors to different extents. The pattern of such inhibition was similar, but not identical, for the receptors induced in hypophysectomized rats and those from pregnant rat liver. The evidence available suggests that the lactogenic receptors induced by bGH-monoclonal antibody complexes are not directly involved in the enhancement of growth.
与仅用牛生长激素（bGH）治疗的动物相比，在12天的治疗期内，用牛生长激素 - 单克隆抗体复合物治疗的雄性垂体切除大鼠体重增加更为明显。从体重增加明显的动物制备的肝微粒体显示人生长激素（hGH）的特异性结合增加。对这些结合位点特异性的研究表明它们具有催乳活性，125I标记的hGH可被绵羊催乳素取代，但不能被非哺乳动物生长激素取代。在这方面，它们类似于妊娠大鼠肝脏中的催乳活性结合位点。抗hGH单克隆抗体在不同程度上阻断了与催乳素受体的结合。对于垂体切除大鼠诱导的受体和妊娠大鼠肝脏中的受体，这种抑制模式相似但不完全相同。现有证据表明，bGH - 单克隆抗体复合物诱导的催乳素受体与生长增强没有直接关系。
