Institute of Ecology and Evolutionary Biology, National Taiwan University, No 1, Sec 4, Roosevelt Road, Taipei 106, Taipei, Taiwan.
Biofouling. 2013;29(2):133-45. doi: 10.1080/08927014.2012.753061.
The present study used DNA barcodes to identify individual cyprids to species. This enables accurate quantification of larvae of potential fouling species in the plankton. In addition, it explains the settlement patterns of barnacles and serves as an early warning system of unwanted immigrant species. Sequences from a total of 540 individual cypris larvae from Taiwanese waters formed 36 monophyletic clades (species) in a phylogenetic tree. Of these clades, 26 were identified to species, but 10 unknown monophyletic clades represented non-native species. Cyprids of the invasive barnacle, Megabalanus cocopoma, were identified. Multivariate analysis of antennular morphometric characters revealed three significant clusters in a nMDS plot, viz. a bell-shaped attachment organ (most species), a shoe-shaped attachment organ (some species), and a spear-shaped attachment organ (coral barnacles only). These differences in attachment organ structure indicate that antennular structures interact directly with the diverse substrata involved in cirripede settlement.
本研究使用 DNA 条码来鉴定浮游幼虫的个体物种。这可以准确地量化潜在污损物种的幼虫数量。此外，它还解释了藤壶的定居模式，并作为一种对不受欢迎的外来物种的预警系统。从台湾水域采集的总共 540 个单体幼虫的序列在系统发育树中形成了 36 个单系群（物种）。在这些单系群中，有 26 个被鉴定为物种，但有 10 个未知的单系群代表非本地物种。鉴定出了入侵性藤壶 Megabalanus cocopoma 的幼虫。触角形态特征的多元分析在 nMDS 图中显示出三个显著的聚类，即钟形附着器官（大多数物种）、鞋形附着器官（一些物种）和矛头状附着器官（仅珊瑚藤壶）。附着器官结构的这些差异表明，触角结构与藤壶定居所涉及的各种基质直接相互作用。
