Winters W D
Vet Rec. 1979 Sep 8;105(10):216-20. doi: 10.1136/vr.105.10.216.
Serum specimens from 42 normal dogs and 42 with untreated malignant tumours were assayed for the presence of antibodies to human adenovirus types 5, 21 and 31 and to infectious canine hepatitis (ICH) virus. Radioimmunoassays using human adenovirus antigens showed that 71 per cent (30/42) of all dogs with tumours, but only 19 per cent (8/42) of all normal dogs, were positive for human adenovirus antibody. Canine sera reactive with antigens of one human adenovirus type in radioimmunoassays were also reactive with antigens of the other two types. Dogs bearing malignant lymphoma or squamous cell carcinoma tumours had higher levels of antibody against adenovirus type 5 antigens. Human adenovirus type 5 was neutralised by sera from four tumour-bearing and two normal dogs, while sera from two normal and five tumour-bearing dogs were positive in immunodiffusion tests with human adenovirus antigens. Levels of ICH antibody in sera of normal adult dogs and adult dogs with tumours were not markedly different when measured by radioimmunoassays. Likewise, sera from these two groups of dogs had similar ranges of ICH neutralising antibody titres. In contrast, levels of ICH antibody detected by the serological assays in sera from non-pet, non-vaccinated pups were either markedly low or absent. Possible explanations for the observed increased levels of human adenovirus antibody in sera of tumour-bearing canine pets are discussed.
对42只正常犬和42只未经治疗的患有恶性肿瘤的犬的血清样本进行检测，以确定是否存在针对人5型、21型和31型腺病毒以及传染性犬肝炎（ICH）病毒的抗体。使用人腺病毒抗原的放射免疫分析表明，所有患肿瘤犬中有71%（30/42）人腺病毒抗体呈阳性，但所有正常犬中只有19%（8/42）呈阳性。在放射免疫分析中与一种人腺病毒抗原发生反应的犬血清，也与其他两种类型的抗原发生反应。患有恶性淋巴瘤或鳞状细胞癌肿瘤的犬，针对5型腺病毒抗原的抗体水平更高。4只患肿瘤犬和2只正常犬的血清可中和人5型腺病毒，而2只正常犬和5只患肿瘤犬的血清在用人类腺病毒抗原进行免疫扩散试验时呈阳性。通过放射免疫分析测定，正常成年犬和患肿瘤成年犬血清中的ICH抗体水平没有明显差异。同样，这两组犬的血清中ICH中和抗体滴度范围相似。相比之下，在非宠物、未接种疫苗的幼犬血清中，通过血清学检测检测到的ICH抗体水平要么明显较低，要么不存在。文中讨论了在患肿瘤的犬类宠物血清中观察到的人腺病毒抗体水平升高的可能原因。
