Sun Cheng-cheng, Jiang Zi-dong, Zhang Kai
Department of Otorhinolaryngology, Peking Union Medical College Hospital, Beijing, China.
Zhonghua Yi Xue Za Zhi. 2012 Dec 25;92(48):3442-4.
To employ micro-CT equipment for nondestructive three-dimensional (3D) imaging of internal ear.
The guinea pigs were anesthetized by napental and bilateral cochleas harvested. Cochlea was fixed in glutaraldehyde before scanning of micro-CT. Two-dimensional (2D) images were acquired for a 3D model of reconstruction.
The 2D images was distinct enough to visualize vestibular gallery, scala media, scala tympani, Reissner's membrane, velum, organ of Corti and spiral ganglion, etc. The 3D structure model was excellent for viewing and free to revolve in any axial direction.
Micro-CT may allow nondestructive three-dimensional imaging of internal ear. As compared with the traditional method of morphology, this approach is able to save samples, easy to operate and has a high resolution. And it is more easily popularized than the synchrotron radiation approach.
采用微型计算机断层扫描（micro-CT）设备对内耳进行无损三维成像。
用戊巴比妥麻醉豚鼠，然后摘取双侧耳蜗。在进行micro-CT扫描前，将耳蜗固定于戊二醛中。采集二维（2D）图像以重建三维模型。
二维图像清晰，足以显示前庭阶、中阶、鼓阶、Reissner膜、前庭膜、柯蒂氏器和螺旋神经节等结构。三维结构模型便于观察，可在任意轴向上自由旋转。
Micro-CT可实现内耳的无损三维成像。与传统形态学方法相比，该方法能够节省样本、操作简便且分辨率高。并且比同步辐射方法更易于推广。
