Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot, United Kingdom.
PLoS One. 2012;7(4):e33568. doi: 10.1371/journal.pone.0033568. Epub 2012 Apr 2.
Imaging with hard X-rays allows visualizing cochlear structures while maintaining intrinsic qualities of the tissue, including structure and size. With coherent X-rays, soft tissues, including membranes, can be imaged as well as cells making use of the so-called in-line phase contrast. In the present experiments, partially coherent synchrotron radiation has been used for micro-tomography. Three-dimensional reconstructions of the mouse cochlea have been created using the EM3D software and the volume has been segmented in the Amira Software Suite. The structures that have been reconstructed include scala tympani, scala media, scala vestibuli, Reissner's membrane, basilar membrane, tectorial membrane, organ of Corti, spiral limbus, spiral ganglion and cochlear nerve. Cross-sectional areas of the scalae were measured. The results provide a realistic and quantitative reconstruction of the cochlea.
硬 X 射线成像是在保持组织固有特性（包括结构和大小）的情况下对耳蜗结构进行可视化的方法。利用相干 X 射线，可以对包括膜在内的软组织以及利用所谓的线内相位对比的细胞进行成像。在本实验中，部分相干同步加速器辐射被用于微断层扫描。使用 EM3D 软件对小鼠耳蜗进行了三维重建，并在 Amira 软件套件中对体积进行了分割。重建的结构包括鼓阶、中阶、前庭阶、Reissner 膜、基底膜、盖膜、Corti 器、螺旋缘、螺旋神经节和耳蜗神经。测量了各阶的横截面积。结果提供了耳蜗的真实和定量重建。
