Savoia D, Malcangi A, Martinetto P
Institute of Microbiology, University of Turin, Italy.
J Chemother. 1990 Feb;2(1):20-5. doi: 10.1080/1120009x.1990.11738975.
Cell surface hydrophobicity is currently regarded as an important factor in promoting bacterial adherence to a wide variety of surfaces. This feature was investigated in some Gram-negative bacteria isolated from urinary tract infections and the extent to which their surface characteristics were affected by subinhibitory concentrations of some antibiotics was assayed. Surface properties were evaluated using the salting-out technique (SAT) and bacterial absorption to n-hexadecane (BATH). SAT showed that all except 3 Escherichia coli strains were autoaggregating. BATH detected more hydrophobic characteristics in the stationary phase of bacterial growth. Pretreatment with antibiotics generally reduced hydrophobicity and thus affected the initial reversible phase of attachment of bacteria to eukaryotic cells.
细胞表面疏水性目前被认为是促进细菌黏附于多种表面的一个重要因素。对从尿路感染中分离出的一些革兰氏阴性菌的这一特性进行了研究，并测定了亚抑菌浓度的某些抗生素对其表面特性的影响程度。使用盐析技术（SAT）和细菌对正十六烷的吸附（BATH）来评估表面性质。SAT显示，除3株大肠杆菌外，所有菌株都能自动聚集。BATH检测到细菌生长稳定期有更多的疏水特性。抗生素预处理通常会降低疏水性，从而影响细菌与真核细胞附着的初始可逆阶段。
