Nuclear Fuel Cycle Research School, Nuclear Science & Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran.
Anal Chim Acta. 2013 Jan 31;762:54-60. doi: 10.1016/j.aca.2012.10.030. Epub 2012 Oct 24.
In this work, a rapid, simple and efficient homogeneous liquid-liquid microextraction via flotation assistance (HLLME-FA) method was developed based on applying low density organic solvents without no centrifugation. For the first time, a special extraction cell was designed to facilitate collection of the low-density solvent extraction in the determination of four polycyclic aromatic hydrocarbons (PAHs) in water samples followed by gas chromatography-flame ionization detector (GC-FID). The effect of different variables on the extraction efficiency was studied simultaneously using experimental design. The variables of interest in the HLLME-FA were selected as extraction and homogeneous solvent volumes, ionic strength and extraction time. Response surface methodology (RSM) was applied to investigate the optimum conditions of all the variables. Using optimized variables in the extraction process, for all target PAHs, the detection limits, the precisions and the linearity of the method were found in the range of 14-41 μg L(-1), 3.7-10.3% (RSD, n=3) and 50-1000 μg L(-1), respectively. The proposed method has been successfully applied to the analysis of four target PAHs in the water samples, and satisfactory results were obtained.
在这项工作中，我们开发了一种基于应用低比重有机溶剂且无需离心的快速、简单、高效的浮选辅助均相液-液微萃取（HLLME-FA）方法。首次设计了一种特殊的萃取池，以方便在气相色谱-火焰离子化检测器（GC-FID）测定水样中的四种多环芳烃（PAHs）时收集低密度溶剂萃取物。同时使用实验设计研究了不同变量对萃取效率的影响。在 HLLME-FA 中，感兴趣的变量选择为萃取和均相溶剂体积、离子强度和萃取时间。响应面法（RSM）用于研究所有变量的最佳条件。在萃取过程中使用优化的变量，对于所有目标 PAHs，方法的检测限、精密度和线性范围分别为 14-41μg/L、3.7-10.3%（RSD，n=3）和 50-1000μg/L。该方法已成功应用于水样中四种目标 PAHs 的分析，得到了满意的结果。
