Lynch D R, Braas K M, Hutton J C, Snyder S H
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
J Neurosci. 1990 May;10(5):1592-9. doi: 10.1523/JNEUROSCI.10-05-01592.1990.
Carboxypeptidase E (CPE, enkephalin convertase, carboxypeptidase H; EC 3.4.17.10) is a carboxypeptidase B-like enzyme proposed to be involved in the synthesis of a variety of peptide hormones and neurotransmitters. In the present study we have localized CPE in the rat central nervous system using specific polyclonal antisera to the purified enzyme. Immunoreactive CPE occurs in a variety of cell somas and neuronal terminals in the central nervous system. The enzyme is most concentrated in the hypothalamus, especially in the median eminence, supraoptic nucleus, paraventricular nucleus, and suprachiasmatic nucleus. CPE is also found in axon terminals in the posterior pituitary gland, melanotropes of the intermediate pituitary gland, and select cells of the anterior pituitary gland. Immunoreactivity to CPE in the hippocampus is found in the pyramidal cells and in the inner part of the molecular layer of the dentate gyrus. CPE is also found in the central nucleus of the amygdala and the bed nucleus of the stria terminalis. These results suggest that CPE distribution corresponds to that of many neuropeptides at the fine structural level, thus further implicating CPE in peptide processing.
羧肽酶E（CPE，脑啡肽转换酶，羧肽酶H；EC 3.4.17.10）是一种类似羧肽酶B的酶，被认为参与多种肽类激素和神经递质的合成。在本研究中，我们使用针对纯化酶的特异性多克隆抗血清，在大鼠中枢神经系统中对CPE进行了定位。免疫反应性CPE存在于中枢神经系统的多种细胞体和神经末梢中。该酶在下丘脑中最为集中，尤其是在正中隆起、视上核、室旁核和视交叉上核。CPE也存在于垂体后叶的轴突末梢、垂体中间叶的促黑素细胞以及垂体前叶的特定细胞中。在海马体中，CPE的免疫反应性存在于锥体细胞和齿状回分子层的内部。CPE也存在于杏仁核的中央核和终纹床核中。这些结果表明，在精细结构水平上，CPE的分布与许多神经肽的分布相对应，从而进一步表明CPE参与肽的加工过程。
