Yang Hui-ling, Mei Sheng-lin, Huang Ping, Zhang Yu-mei
Department of Prosthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2012 Dec;47(12):748-52. doi: 10.3760/cma.j.issn.1002-0098.2012.12.013.
To study the effects of ultraviolet ray (UV)-irradiation on the surface characteristic and antibacterial activity of TiO(2) nanotubes with different diameters.
TiO(2) nanotubes with different diameters were fabricated on polished pure titanium (PT) samples by anodization at 5, 10 and 20 V with PT as control. The samples were exposed to UV-irradiation for 24 h, then the characteristic and antibacterial activity were analyzed and evaluated. The surface topograph was observed by field emission scanning electron microscope (FE-SEM). Contract angle measurements were carried out with three liquids. Staphylococcus aureus (Sa) were used to evaluate the antibacterial activity of samples with the film contact method. The bacterial morphology was observed by FE-SEM. The bacterial adhesion and cell membrane injury were evaluated by fluorescent staining analysis under laser scanning confocal microscope (LSCM).
After the TiO(2) nanotubes with different diameters were exposed to UV-irradiation, no change was observed in its surface topograph. With the increase of the diameters of nanotubes, each contract angle of nanotubes decreased, and bacterial FIt and dead/live ratio were also increased. We found 20 V FIt was the biggest (26.550 ± 2.940) and ranks the highest ratio of death/live (0.728 ± 0.091) among the others (P < 0.05).
The UV-irradiation can decrease the contract angle of TiO(2) nanotubes and promote the Sa adhesion on nanotubes. Meanwhile, the antibacterial activity of TiO(2) nanotubes with different diameters was remarkably enhanced by UV-irradiation. Nanotubes anodized at 20 V showed the best antibacterial activity.
研究紫外线（UV）照射对不同直径二氧化钛（TiO₂）纳米管表面特性及抗菌活性的影响。
以抛光纯钛（PT）样品为对照，通过在5V、10V和20V电压下进行阳极氧化制备不同直径的TiO₂纳米管。将样品进行紫外线照射24小时，然后对其特性和抗菌活性进行分析和评估。用场发射扫描电子显微镜（FE-SEM）观察表面形貌。用三种液体进行接触角测量。采用薄膜接触法用金黄色葡萄球菌（Sa）评估样品的抗菌活性。用FE-SEM观察细菌形态。在激光扫描共聚焦显微镜（LSCM）下通过荧光染色分析评估细菌黏附和细胞膜损伤情况。
不同直径的TiO₂纳米管经紫外线照射后，其表面形貌未观察到变化。随着纳米管直径的增加，纳米管的各接触角减小，细菌荧光强度（FIt）和死/活比率也增加。我们发现20V电压下制备的纳米管FIt最大（26.550±2.940），且死/活比率在其他组中最高（0.728±0.091）（P<0.05）。
紫外线照射可降低TiO₂纳米管的接触角，促进金黄色葡萄球菌在纳米管上的黏附。同时，紫外线照射显著增强了不同直径TiO₂纳米管的抗菌活性。20V电压下阳极氧化制备的纳米管抗菌活性最佳。
