Hu Bin, Xu Hong-guang, Song Jun-xing, Zhang Ping-zhi, Zhang Xiao-hai, Cheng Jia-Feng
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.
Zhonghua Yi Xue Za Zhi. 2012 Dec 18;92(47):3341-4.
To observe the expression changes of Sirt1 gene and examine the role and significance of degenerative process in human cervical endplate chondrocytes through a degeneration model of human cervical vertebral endplate chondrocyte.
Cartilage endplates of 30 patients were divided into control group (n = 16) with cervical vertebral fracture or dislocation and cervical spondylosis group (n = 14) with cervical spondylotic myelopathy. Endplate chondrocytes were isolated by enzyme digestion and cultured in vitro for 10 days. The differences of endplate chondrocytes from normal and degenerative cartilage endplates were observed by inverted phase-contrast microscope, hematoxylin and eosin staining and toluidine blue staining. Real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the mRNA expressions of Sirt1, collagen II and aggrecan.
Compared with the normal group, the cellular morphology of degenerative group showed spindle-shaped changes. The mRNA expression of Sirt1 (P = 0.034) significantly decreased. Aggrecan (P = 0.0063) and collagen II (P = 0.0072) decreased also markedly.
Sirt1 gene expression is significantly down-regulated in degenerative human cervical endplate chondrocytes. Regulating the expression of Sirt1 gene may block or delay the occurrence of human cervical endplate cartilage degeneration.
通过人颈椎终板软骨细胞退变模型，观察Sirt1基因的表达变化，探讨其在人颈椎终板软骨细胞退变过程中的作用及意义。
选取30例患者的软骨终板，其中颈椎骨折或脱位患者16例作为对照组，脊髓型颈椎病患者14例作为颈椎病组。采用酶消化法分离终板软骨细胞并进行体外培养10天。通过倒置相差显微镜、苏木精-伊红染色和甲苯胺蓝染色观察正常和退变软骨终板来源的终板软骨细胞的差异。采用实时逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测Sirt1、Ⅱ型胶原和聚集蛋白聚糖的mRNA表达。
与正常组相比，退变组细胞形态呈梭形改变。Sirt1的mRNA表达显著降低（P = 0.034）。聚集蛋白聚糖（P = 0.0063）和Ⅱ型胶原（P = 0.0072）也明显降低。
在退变的人颈椎终板软骨细胞中，Sirt1基因表达显著下调。调节Sirt1基因的表达可能会阻止或延缓人颈椎终板软骨退变的发生。
