Department of Applied Chemistry, National University of Kaohsiung, 700 Kaohsiung University Road, Kaohsiung 811, Taiwan.
Chem Commun (Camb). 2013 Mar 25;49(24):2397-9. doi: 10.1039/c2cc38233d.
Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.
本文介绍了一种独特且廉价的方法，优于放大链霉亲和素-生物素识别事件的商业方法。链霉亲和素和生物素化蛋白诱导的扩增导致形成可检测的聚合物。与商业方法相比，这种方法的检测限降低了 100 倍。
