Cuchiara Michael P, West Jennifer L
Department of Bioengineering, MS-142, BRC, Rice University, Houston, TX, USA.
Methods Mol Biol. 2013;949:387-401. doi: 10.1007/978-1-62703-134-9_24.
Development of robust, in vivo like tissues in vitro holds the potential to create regenerative medicine-based therapeutics, provide more physiologically significant preclinical models and supply a pharmacological and toxicological screening platform that reflects in vivo systems in both complexity and function. This protocol describes a simple, robust, multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings, using a combination of soft and photo-lithography. This work has direct applications toward the development of robust, complex, cell-laden hydrogels for in vitro diagnostics and regenerative medicine applications.
在体外培养出强健的、类似体内组织的结构，有望开发出基于再生医学的疗法，提供更具生理意义的临床前模型，并搭建一个在复杂性和功能上都能反映体内系统的药理和毒理学筛选平台。本方案描述了一种简单、可靠的多层复制成型技术，其中聚二甲基硅氧烷（PDMS）和聚乙二醇二丙烯酸酯（PEGDA）通过软光刻和光光刻相结合的方式进行连续复制成型，以开发嵌入独立制造的PDMS外壳内的微流控PEGDA水凝胶网络。这项工作对于开发用于体外诊断和再生医学应用的强健、复杂、负载细胞的水凝胶具有直接应用价值。
