Basler Michael, Groettrup Marcus
Biotechnology Institute Thurgau at the University of Constance, Kreuzlingen, Switzerland.
Department of Biology, Division of Immunology, University of Konstanz, Konstanz, Germany.
Methods Mol Biol. 2013;960:31-39. doi: 10.1007/978-1-62703-218-6_3.
The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.
主要组织相容性复合体（MHC）I类限制的抗原加工途径可将细胞内抗原呈递给细胞毒性T淋巴细胞。蛋白酶体是细胞质和细胞核中的主要蛋白酶，负责生成大多数MHC-I分子的肽配体。蛋白酶体产生的肽可被多种胞质或内质网（ER）腔蛋白酶进一步修剪或破坏。小分子抑制剂是探究蛋白酶在MHC I类抗原加工中作用的有用工具。在此，我们描述了在抗原呈递试验中测试蛋白酶抑制剂影响的不同方法。
