China Minority Traditional Medical Institute, Minzu University of China, Beijing, China.
Eur Rev Med Pharmacol Sci. 2013 Jan;17(1):88-94.
Vascular endothelium injury caused by reactive oxygen species (ROS) plays an initial role in the pathogenesis of atherosclerosis. Resveratrol, a natural polyphenolic compound, is well known to possess a variety of cardioprotective activities. In this study we first investigated the effects of resveratrol against apoptosis of human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide (H2O2) in vitro and the possible mechanisms.
HUVECs were pre-incubated with resveratrol (0.5-10 microM) for 120 min, and then challenged with 100 microM H2O2 for 30 min. The cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The superoxide dismutase (SOD) activities and the reduced glutathione (GSH) contents were measured spectrophotometrically by using commercially available kits. The apoptosis of HUVECs was detected by Hoechst 33258 and Annexin-V/PI staining by flow cytometry staining using Fluorescence microscope. We also measured the mitochondrial membrane potential with the fluorescent probe JC-1 through Fluorescence microscope.
The study showed that incubation with resveratrol caused significant increase of the viability of HUVECs and the SOD activities and GSH contents, and decrease of cell apoptosis induced by H2O2, which was accompanied with the restoration of the mitochondrial membrane potential.
Our data suggested that resveratrol protected HUVECs against apoptosis induced by H2O2 through enhancing the antioxidant defenses, and inhibiting the degression of the mitochondrial membrane potential.
活性氧（ROS）引起的血管内皮损伤在动脉粥样硬化发病机制中起初始作用。白藜芦醇是一种天然多酚化合物，具有多种心脏保护作用。在这项研究中，我们首先研究了白藜芦醇对体外过氧化氢（H2O2）诱导的人脐静脉内皮细胞（HUVEC）凋亡的作用及其可能的机制。
将 HUVEC 用白藜芦醇（0.5-10 μM）预孵育 120 分钟，然后用 100 μM H2O2 处理 30 分钟。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法评估细胞活力。通过使用市售试剂盒分光光度法测量超氧化物歧化酶（SOD）活性和还原型谷胱甘肽（GSH）含量。通过荧光显微镜用 Hoechst 33258 和 Annexin-V/PI 染色通过流式细胞术染色检测 HUVEC 凋亡。我们还通过荧光显微镜用荧光探针 JC-1 测量线粒体膜电位。
研究表明，白藜芦醇孵育可显著增加 HUVEC 的活力和 SOD 活性和 GSH 含量，并降低 H2O2 诱导的细胞凋亡，同时恢复线粒体膜电位。
我们的数据表明，白藜芦醇通过增强抗氧化防御和抑制线粒体膜电位下降来保护 HUVEC 免受 H2O2 诱导的凋亡。
