长期接触汞的个体中，HSP70和HSP90血液水平与HSP70、GSTT1和GSTM1基因多态性基因型的相关性。

Dependence of blood levels of HSP70 and HSP90 on genotypes of HSP70, GSTT1, and GSTM1 gene polymorphism in individuals chronically exposed to mercury.

作者信息

Chernyak Yu I, Itskovich V B, Baduev B K, Borovskii G B

机构信息

Angarsk [corrected] Affiliated Department of East Siberian Center of Human Ecology, Siberian Division of the Russian Academy of Medical Sciences, Irkutsk, Russia.

出版信息

Bull Exp Biol Med. 2012 Nov;154(1):68-72. doi: 10.1007/s10517-012-1877-3.

DOI:10.1007/s10517-012-1877-3

PMID:23330093

Abstract

The relationship between blood levels of HSP72, HSP72+HSP73, and HSP90 and genotypes of three polymorphisms of the HSP70 family, HSPA1L (2437T/C) and HSPA1B (2074G/C and 1267A/G) as well as GSTT1 and GSTM1 polymorphisms was studied in 82 men chronically exposed to mercury. Of these, 40 men were exposed to mercury for more than 10 years (group 1) and 42 developed chronic mercuric intoxication (group 2). The groups differed significantly by TT (p=0.004) and TC (p=0.007) genotypes of HSPA1L gene locus 2437T/C. Differences in the heat shock protein content associated with HSP70 gene polymorphism were detected only for HSPA1B gene locus 2074G/C and consisted in reduction of HSP90 (p=0.020) and HSP72 (p=0.056) for GG genotype in group 2 in comparison with group 1. Combination of GSTT1(+)/GSTM1(0/0) genotypes was associated with reduction of the protein levels, while variants including GSTT1(0/0) were associated with a significant elevation thereof.

摘要

在82名长期接触汞的男性中，研究了热休克蛋白72（HSP72）、热休克蛋白72 + 热休克蛋白73（HSP72 + HSP73）和热休克蛋白90（HSP90）的血液水平与热休克蛋白70家族三个多态性位点（即HSPA1L基因2437T/C、HSPA1B基因2074G/C和1267A/G）的基因型以及谷胱甘肽S-转移酶T1（GSTT1）和谷胱甘肽S-转移酶M1（GSTM1）多态性之间的关系。其中，40名男性接触汞超过10年（第1组），42名患有慢性汞中毒（第2组）。HSPA1L基因位点2437T/C的TT基因型（p = 0.004）和TC基因型（p = 0.007）在两组之间存在显著差异。仅在HSPA1B基因位点2074G/C检测到与热休克蛋白70基因多态性相关的热休克蛋白含量差异，表现为第2组GG基因型的HSP90（p = 0.020）和HSP72（p = 0.056）相较于第1组降低。GSTT1(+)/GSTM1(0/0)基因型组合与蛋白水平降低相关，而包括GSTT1(0/0)的变体则与蛋白水平显著升高相关。