个体发育哺乳期实验性扩大大鼠脑新皮层和海马的形态测量学及组织化学特征

Morphometric and histochemical characteristics of the neocortex and hippocampus of experimentally enlarged rat brain during the suckling period of ontogeny.

作者信息

Ryzhavskii B Ya, Litvintseva E M

机构信息

Department of Histology, Far-Eastern State Medical University, Khabarovsk, Russia.

出版信息

Bull Exp Biol Med. 2012 Nov;154(1):96-9. doi: 10.1007/s10517-012-1884-4.

DOI:10.1007/s10517-012-1884-4

PMID:23330100

Abstract

Enlargement of the brain weight was attained in rats by reducing the number of neonates in the litter. The brain of 14- and 30-day-old animals was examined (controls: intact animals from litters of average numbers). Difference between the brain weight at 14 and 30 days was about 200 mg. The parameter in the experimental rats was higher than in control: by 19.9 and 15.8% at the age of 14 and 30 days, respectively. The neocortex and hippocampus of the experimental rat brain differed by the size of the neuron cytoplasm and nucleus, these cells' count in a visual field, and activities of NADH- and NADPH-dehydrogenases in the neuron cytoplasm. During the suckling period, the differences in the weights of experimentally enlarged brain were paralleled by specific features of the cortex, morphometric and histochemical characteristics of the neurons.

摘要

通过减少每窝新生大鼠的数量，使大鼠脑重量增加。对14日龄和30日龄动物的大脑进行了检查（对照组：来自平均数量窝的完整动物）。14天和30天时脑重量的差异约为200毫克。实验大鼠的该参数高于对照组：在14日龄和30日龄时分别高出19.9%和15.8%。实验大鼠脑的新皮层和海马体在神经元细胞质和细胞核的大小、视野中这些细胞的数量以及神经元细胞质中NADH和NADPH脱氢酶的活性方面存在差异。在哺乳期，实验性增大的脑重量差异与皮层的特定特征、神经元的形态计量学和组织化学特征平行。