缺氧对人孤雌生殖干细胞集落神经诱导的影响。

Effect of hypoxia on neural induction in colonies of human parthenogenetic stem cells.

作者信息

Abramihina T V, Isaev D A, Semechkin R A

机构信息

International Stem Cell Corporation, Carlsbad, USA.

出版信息

Bull Exp Biol Med. 2012 Nov;154(1):130-2. doi: 10.1007/s10517-012-1892-4.

DOI:10.1007/s10517-012-1892-4

PMID:23330108

Abstract

We studied neural induction and generation of neuroectoderm in the colonies of human parthenogenetic SC cultured in the presence of 5 and 19±2% oxygen. We found that neuroectoderm was more actively generated at high oxygen content. At the same time, the transcription of stem cell pluripotency genes was not completely suppressed during neural induction at low oxygen content, while the expression of endoderm and mesodermal marker genes attested to the absence of specific differentiation. These findings demonstrate more efficient neuroectoderm generation induced in the colonies of pluripotent stem cells under conditions of normoxia.

摘要

我们研究了在5%和19±2%氧气存在下培养的人孤雌生殖干细胞集落中的神经诱导和神经外胚层的产生。我们发现，在高氧含量下神经外胚层的产生更为活跃。同时，在低氧含量的神经诱导过程中，干细胞多能性基因的转录并未被完全抑制，而内胚层和中胚层标记基因的表达表明不存在特异性分化。这些发现证明了在常氧条件下多能干细胞集落中诱导产生神经外胚层的效率更高。