Dolgikh O V, Agafonov Iu V, Zashikhin A L
Morfologiia. 2012;142(5):59-63.
The myometrium of various parts of uterine wall was studied in 40 albino rats--non-pregnant animals, rats at different stages of pregnancy and after parturition. The methods of immunocytochemistry (detection of apoptotic cells with the help of antibodies against the tumor necrosis factor receptor), complex morphometic and cytochemical analysis of DNA content in the nuclei and of total protein in the cytoplasm of isolated smooth-myocytes (SM). The regularities of structural reconstruction of SM population in the uterine wall at various physiological periods were detected. The realization of basic mechanisms of myometrial transformation during the pregnancy, such as hyperplasia and hypertrophy are different in various uterine parts because of their specialized functional load. At the early stages of pregnancy, small SM were found to prevail in SM populations of all the uterine part, proliferative potential was increased. By the end of gestation, the fraction of large-sized SM was increased in the horns, the synthetic processes in the cytoplasm were activated, while the fraction of medium-sized SM was increased in the corpus and the cervix. The structure of SM population in the horns on Day 7 of postpartum involution was similar to that of non-pregnant rats, in the corpus and the cervix the increase of small SM number was observed.
对40只白化大鼠子宫壁各部分的肌层进行了研究，这些大鼠包括未怀孕的动物、处于不同妊娠阶段的大鼠以及产后大鼠。采用免疫细胞化学方法（借助抗肿瘤坏死因子受体抗体检测凋亡细胞）、对分离的平滑肌细胞（SM）细胞核中的DNA含量和细胞质中的总蛋白进行复杂的形态计量和细胞化学分析。检测了子宫壁在不同生理时期SM群体结构重建的规律。由于子宫各部分的特殊功能负荷，妊娠期间子宫肌层转化的基本机制，如增生和肥大，在不同子宫部位有所不同。在妊娠早期，所有子宫部位的SM群体中以小SM为主，增殖潜能增加。到妊娠末期，角部大尺寸SM的比例增加，细胞质中的合成过程被激活，而体部和宫颈中中等尺寸SM的比例增加。产后复旧第7天角部SM群体的结构与未怀孕大鼠相似，体部和宫颈中小SM数量增加。
