College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, South Korea.
J Ethnopharmacol. 2013 Mar 7;146(1):347-54. doi: 10.1016/j.jep.2013.01.003. Epub 2013 Jan 16.
The fruits of Schisandra chinensis (Trucz.) Baill. (Schisandraceae) which have been used as a tonic especially for kidney yin deficiency in Chinese traditional medicine are recently receiving attention for its preventive activity on age-related neurodegenerative diseases. A variety of studies demonstrated the cognitive-enhancing effects of Schisandra chinensis through animal tests and also in clinical trials.
In this study, we attempted to investigate the effects of the lignan-riched extract of Schisandra chinensis fruits (ESP-806) on neurotoxicity and memory impairment induced by Aβ1-42 injection in mice.
The fruits of Schisandra chinensis were extracted with the mixture of n-hexane:ethanol (9:1), which is riched with bioactive dibenzocyclooctadiene lignans, schizandrin, gomisin N, wuweigisu C. After oral treatment of ESP-806 (100 mg/kg body weight) followed by injection of Aβ1-42 (2 μg/mouse, i.c.v.), novel object recognition and passive avoidance tests were evaluated. To verify the cognition enhancing effects of ESP-806, we examined the effects of ESP-806 on the activities of β-secretase and acetylcholinesterase, and the contents of Aβ and the reduced glutathione within the cortex and hippocampus of Aβ-injected mice.
Oral treatment of ESP-806 (100 mg/kg body weight) significantly attenuated Aβ1-42-induced memory impairment evaluated by behavioral tests. Furthermore, the treatment of ESP-806 attenuated the elevation of β-secretase activity accompanying the reduced level of Aβ1-42 in the cortex and hippocampus of the brain. ESP-806 also significantly inhibited the acetylcholinesterase activity in the hippocampus and increased the content of the reduced glutathione in the cortex and hippocampus of mouse brain.
These data suggested that the extract of Schisandra chinensis fruits riched with dibenzocyclooctadiene lignans may be useful in the prevention and treatment of Alzheimer's disease.
五味子（Trucz.）Baill。（五味子科）的果实一直被用作中药中的滋补品，特别是用于治疗肾阴虚。五味子的各种研究通过动物试验和临床试验证明了其对与年龄相关的神经退行性疾病的预防作用。
在这项研究中，我们试图研究五味子果实木质素丰富提取物（ESP-806）对 Aβ1-42 注射诱导的小鼠神经毒性和记忆障碍的影响。
用正己烷：乙醇（9：1）混合物提取五味子果实，该混合物富含生物活性的二苯并环辛二烯木质素，五味子素、五味子甲素、五味子乙素。口服 ESP-806（100mg/kg 体重）后，注射 Aβ1-42（2μg/只，脑室内），进行新物体识别和被动回避试验。为了验证 ESP-806 的认知增强作用，我们研究了 ESP-806 对β-分泌酶和乙酰胆碱酯酶活性以及 Aβ含量和还原型谷胱甘肽含量的影响。
ESP-806（100mg/kg 体重）口服治疗显著减轻了行为测试评估的 Aβ1-42 诱导的记忆障碍。此外，ESP-806 治疗降低了皮质和海马中 Aβ1-42 水平伴随的β-分泌酶活性升高。ESP-806 还显著抑制了海马中的乙酰胆碱酯酶活性，并增加了小鼠脑皮质和海马中还原型谷胱甘肽的含量。
这些数据表明，富含二苯并环辛二烯木质素的五味子果实提取物可能有助于预防和治疗阿尔茨海默病。
