Max-Planck Institute for Terrestrial Microbiology; Marburg, Germany.
Plant Signal Behav. 2013 Apr;8(4):e23572. doi: 10.4161/psb.23572. Epub 2013 Jan 18.
Beside a cardinal role in coordination of many developmental processes in the plant, the phytohormone auxin has been recognized as a regulator of plant defense. The molecular mechanisms involved are still largely unknown. Using a sensitive chemiluminescence assay, which measures the oxidation of luminol in the presence of H₂O₂ by horseradish peroxidase (HRP), we report here on the ability of exogenously added indole-3-acetic acid (IAA) to enhance the suppressive effect of the root endophyte Piriformospora indica on the chitin-elicited oxidative burst in barley roots. Thus, the potential of P. indica to produce free IAA during the early colonization phase in barley might provide the symbiont with a means to interfere with the microbe-associated molecular patterns (MAMP)-triggered immunity.
除了在植物的许多发育过程中发挥核心作用外，植物激素生长素也被认为是植物防御的调节剂。但其中涉及的分子机制在很大程度上仍然未知。本研究使用一种灵敏的化学发光测定法，该方法通过辣根过氧化物酶（HRP）在 H₂O₂存在下测量鲁米诺的氧化，报告了外源性添加吲哚-3-乙酸（IAA）增强根内寄生菌印楝（Piriformospora indica）对大麦根几丁质诱导的氧化爆发的抑制作用的能力。因此，印楝在大麦早期定植阶段产生游离 IAA 的能力可能为共生体提供了一种干扰微生物相关分子模式（MAMP）触发免疫的手段。
