Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440020, India.
Bioresour Technol. 2013 Feb;130:644-51. doi: 10.1016/j.biortech.2012.12.051. Epub 2012 Dec 20.
An enriched biomass was developed from municipal sewage sludge consisting of three dominant bacteria, representing the genera of Enterobacter, Citrobacter and Streptomyces. The biomass was used in a series of batch experiments in order to determine kinetic constants associated with biomass growth and NOx reduction in aqueous Ferrous EDTA/NTA solutions and Ferric EDTA/NTA solutions using ethanol as organic electron donor. The maximum specific reduction rates of NOx in Ferrous EDTA and Ferrous NTA solutions were 0.037 and 0.047mMolesL(-1)d(-1)mg(-1) biomass, respectively while in Ferric EDTA and Ferric NTA solutions were 0.022 and 0.024mMolesL(-1)d(-1)mg(-1) biomass, respectively. In case of Ferric EDTA/NTA solution, the kinetic constants associated with reduction of Ferric EDTA/NTA to Ferrous EDTA/NTA were also evaluated simultaneously. The maximum specific reduction rates of Ferric EDTA and Ferric NTA were 0.0021 and 0.0026mMolesL(-1)d(-1)mg(-1) biomass. The significance of these observations are presented and discussed in this paper.
从城市污水污泥中开发出富含生物质的物质,其中包含三种主要细菌,分别代表肠杆菌属、柠檬酸杆菌属和链霉菌属。为了确定在含有乙醇作为有机电子供体的亚铁 EDTA/NTA 溶液和高铁 EDTA/NTA 溶液中,生物量生长和 NOx 还原相关的动力学常数,使用该生物质进行了一系列分批实验。亚铁 EDTA 和亚铁 NTA 溶液中 NOx 的最大比还原速率分别为 0.037 和 0.047mMolesL(-1)d(-1)mg(-1)生物质,而在高铁 EDTA 和高铁 NTA 溶液中分别为 0.022 和 0.024mMolesL(-1)d(-1)mg(-1)生物质。在高铁 EDTA/NTA 溶液的情况下,还同时评估了将高铁 EDTA/NTA 还原为亚铁 EDTA/NTA 的动力学常数。高铁 EDTA 和高铁 NTA 的最大比还原速率分别为 0.0021 和 0.0026mMolesL(-1)d(-1)mg(-1)生物质。本文提出并讨论了这些观察结果的意义。