Nosi D, Delfino G, Quercioli F
Department of Anatomy, Histology, and Forensic Medicine, University of Florence, Florence, Italy.
Naturwissenschaften. 2013 Mar;100(3):209-18. doi: 10.1007/s00114-013-1013-x. Epub 2013 Jan 20.
A combined transmission electron microscopy (TEM) and Fourier transform analysis has been performed on the secretory granules storing active peptides/proteins in serous cutaneous glands of n = 12 anuran species. Previous TEM investigation showed that the granules are provided with remarkable repeating substructures based on discrete subunits, arranged into a consistent framework. Furthermore, TEM findings revealed that this recurrent arrangement is acquired during a prolonged post-Golgian (or maturational) processing that affects the secretory product. Maturation leads to a variety of patterns depending on the degree of subunit clustering. This variety of recurrent patterns has been plotted into a range of frequency spectra. Through this quantitative approach, we found that the varying granule substructure can be reduced to a single mechanism of peptide/protein aggregation.
对12种无尾目动物浆液性皮肤腺中储存活性肽/蛋白质的分泌颗粒进行了透射电子显微镜（TEM）和傅里叶变换分析。先前的TEM研究表明，这些颗粒具有基于离散亚基的显著重复亚结构，排列成一个一致的框架。此外，TEM研究结果显示，这种重复排列是在影响分泌产物的长时间高尔基体后（或成熟）加工过程中获得的。成熟会根据亚基聚集程度导致多种模式。这种多种重复模式已被绘制到一系列频谱中。通过这种定量方法，我们发现不同的颗粒亚结构可以简化为肽/蛋白质聚集的单一机制。
