College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Jeonbuk 570-749, Republic of Korea.
Am J Chin Med. 2013;41(1):71-83. doi: 10.1142/S0192415X13500067.
Mesangial cell proliferation is correlated with the progression of renal failure. The purpose of this study was to determine whether a water extract of Poria cocos Wolf (WPC), a well-known medicinal plant, regulates rat mesangial cell proliferation in the presence of high glucose (HG). HG significantly accelerated [(3)H]-thymidine incorporation, which was inhibited by WPC (1-50 μg/mL) in a dose-dependent manner. Cell migration and fibronectin mRNA expression data also supported the anti-proliferative effect of WPC. Western blot analysis revealed that pretreatment with WPC decreased the expression of cyclins and cyclin-dependent kinases (CDKs) and promoted the expression of p21(waf1/cip1) and p27(kip1). WPC also suppressed HG-induced p38 mitogen-activated protein kinase (p38 MAPK) and extracellular-signal-regulated kinase 1/2 (ERK 1/2) phosphorylation. Furthermore, WPC inhibited HG-induced production of dichlorofluorescein (DCF)-sensitive intracellular reactive oxygen species (ROS). In conclusion, HG promoted mesangial cell proliferation, and WPC inhibited this activity, at least in part, via induction of cell cycle arrest and activation of anti-oxidant properties. Taken together, these results suggest that P. cocos may be a potent regulator of HG-induced proliferation.
系膜细胞增殖与肾衰竭的进展有关。本研究旨在确定一种众所周知的药用植物茯苓（Poria cocos Wolf）的水提取物（WPC）在高葡萄糖（HG）存在的情况下是否能调节大鼠系膜细胞增殖。HG 显著加速了[3H]胸苷的掺入，而 WPC（1-50μg/mL）以剂量依赖的方式抑制了掺入。细胞迁移和纤连蛋白 mRNA 表达数据也支持 WPC 的抗增殖作用。Western blot 分析表明，WPC 预处理可降低细胞周期蛋白和细胞周期蛋白依赖性激酶（CDKs）的表达，促进 p21（waf1/cip1）和 p27（kip1）的表达。WPC 还抑制了 HG 诱导的丝裂原活化蛋白激酶（p38 MAPK）和细胞外信号调节激酶 1/2（ERK 1/2）磷酸化。此外，WPC 抑制了 HG 诱导的二氯荧光素（DCF）敏感细胞内活性氧（ROS）的产生。总之，HG 促进了系膜细胞的增殖，而 WPC 通过诱导细胞周期停滞和激活抗氧化特性至少部分抑制了这种活性。综上所述，这些结果表明，茯苓可能是 HG 诱导增殖的有效调节剂。
