Xuzhou Medical College, Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment, and Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
Reg Anesth Pain Med. 2013 Mar-Apr;38(2):93-9. doi: 10.1097/AAP.0b013e3182795b23.
Little research has been conducted regarding the implications of Sirt1 (a classic III HDAC) in neuropathic pain. The aim of this study was to investigate the variation in the expressions of spinal Sirt1 and acetyl-histone H3 in a rat model of chronic constriction injury.
A neuropathic pain model of chronic constriction injury (CCI) was established in a unilateral hind limb in Sprague-Dawley rats.
Western blot analysis and immunohistochemistry revealed that Sirt1 (silent information regulator) expression decreased, whereas acetyl-histone H3 increased in the spinal cord following CCI surgery. The intrathecal administration of resveratrol, an activator of Sirt1, attenuated CCI-induced mechanical allodynia and thermal hyperalgesia, increased Sirt1 expression, and decreased acetyl-histone H3 in the spine. Resveratrol induced no obvious histopathological changes in the spinal cord.
Our data provide new evidence for the contribution of spinal Sirt1 to the initiation and maintenance of neuropathic pain. The antinociceptive effects of resveratrol may be mediated through the activation of spinal Sirt1 in CCI rats.
关于 Sirt1（一种经典的 III 类组蛋白去乙酰化酶）在神经病理性疼痛中的意义，研究甚少。本研究旨在探讨慢性缩窄性损伤（CCI）大鼠模型中脊髓 Sirt1 和乙酰组蛋白 H3 表达的变化。
在 Sprague-Dawley 大鼠单侧后肢建立神经病理性疼痛的慢性缩窄性损伤（CCI）模型。
Western blot 分析和免疫组化显示，CCI 手术后脊髓 Sirt1（沉默信息调节因子）表达降低，乙酰组蛋白 H3 增加。鞘内给予 Sirt1 激活剂白藜芦醇可减轻 CCI 诱导的机械性痛觉过敏和热痛觉过敏，增加脊髓 Sirt1 表达，降低脊髓乙酰组蛋白 H3。白藜芦醇对脊髓无明显的组织病理学改变。
本研究为脊髓 Sirt1 参与神经病理性疼痛的发生和维持提供了新的证据。白藜芦醇的镇痛作用可能是通过激活 CCI 大鼠的脊髓 Sirt1 介导的。
