Department of General Surgery, Shengjing Hospital of China Medical University, and Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110004, PR China.
Oncol Rep. 2013 Apr;29(4):1556-62. doi: 10.3892/or.2013.2236. Epub 2013 Jan 15.
The S100A2 gene has been reported to be a putative tumor‑suppressor gene. Nevertheless, overexpression of S100A2 has been found in certain types of cancer. This study investigated S100A2 expression in tissue specimens of gastritis, intestinal metaplasia, adenomatous dysplasia and gastric cancer to determine its association with clinical features. A serial of tissue samples (gastritis, intestinal metaplasia, adenomatous dysplasia and gastric cancer samples) were used for quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR), western blotting and immunohistochemical analyses of S100A2 expression. The data revealed that there was a gradual loss of S100A2 expression from gastritis, intestinal metaplasia and dysplasia to cancer tissue specimens (p<0.001). In gastric cancer samples, loss of S100A2 expression was associated with increased tumor size, depth of invasion, lymph node metastasis and a poor prognosis (p<0.001). However, the intestinal type of gastric cancer expressed more S100A2 protein than the diffuse type (p<0.001). In conclusion, data from the present study demonstrated that loss of S100A2 expression contributes to gastric cancer development and progression; therefore, the determination of S100A2 expression levels may help to predict the carcinogenesis and aggressiveness of gastric cancer as well as patient survival.
S100A2 基因已被报道为一种潜在的肿瘤抑制基因。然而，在某些类型的癌症中发现了 S100A2 的过表达。本研究调查了 S100A2 在胃炎、肠上皮化生、腺瘤性发育不良和胃癌组织标本中的表达，以确定其与临床特征的关系。一系列组织样本（胃炎、肠上皮化生、腺瘤性发育不良和胃癌样本）用于 S100A2 表达的定量实时逆转录-聚合酶链反应（qRT-PCR）、western blot 和免疫组织化学分析。数据显示，从胃炎、肠上皮化生和发育不良到癌症组织标本，S100A2 的表达逐渐丧失（p<0.001）。在胃癌样本中，S100A2 表达的丧失与肿瘤大小增加、浸润深度、淋巴结转移和预后不良有关（p<0.001）。然而，肠型胃癌比弥漫型胃癌表达更多的 S100A2 蛋白（p<0.001）。总之，本研究的数据表明，S100A2 表达的丧失有助于胃癌的发生和发展；因此，S100A2 表达水平的测定可能有助于预测胃癌的癌变和侵袭性以及患者的生存。
