Department of Biological and Agricultural Engineering, University of California, One Shields Avenue, Davis, CA, 95616, USA,
Bioprocess Biosyst Eng. 2013 Oct;36(10):1427-34. doi: 10.1007/s00449-013-0886-6. Epub 2013 Jan 22.
Water-in-oil emulsions provide an alternative for long-term stabilization of microorganisms. Maintaining physical stability of the emulsion and cell viability is critical for large-scale application. Water-in-oil (W/O) emulsions were prepared with the biolarvacide Lagenidium giganteum and the green alga Chlorella vulgaris. Physical stability was measured via light scattering measurements of the internal phase droplets and cell viability was measured by plating and enumerating colony forming units. Emulsions were demonstrated to stabilize L. giganteum and C. vulgaris for more than 4 months without refrigeration. Introducing nutrients into the internal phase of W/O emulsions without cells had no significant effect on changes in aqueous phase droplet size dynamics. Internal phase droplet size changes that occurred over time were greater in the presence of cells. Increases in droplet size were correlated with cell death indicating measurement of internal phase droplet size changes may be an approach for monitoring declines in cell viability during storage.
水包油乳液为微生物的长期稳定提供了一种替代方法。保持乳液的物理稳定性和细胞活力对于大规模应用至关重要。使用生物杀虫剂丽蚜小蜂和绿藻普通小球藻制备了水包油（W/O）乳液。通过内部相液滴的光散射测量来测量物理稳定性，通过平板和计数集落形成单位来测量细胞活力。结果表明，乳液在不冷藏的情况下可稳定丽蚜小蜂和普通小球藻超过 4 个月。向无细胞的 W/O 乳液内部相引入营养物质对水相液滴尺寸动态变化没有明显影响。随着时间的推移，内部相液滴尺寸的变化在存在细胞的情况下更大。液滴尺寸的增加与细胞死亡相关，这表明测量内部相液滴尺寸的变化可能是监测储存过程中细胞活力下降的一种方法。
