Alberti Luiz Ronaldo, Vasconcellos Leonardo de Souza, Petroianu Andy
Department of Surgery, Faculty of Medicine, UFMG, Belo Horizonte, MG, Brazil.
Acta Cir Bras. 2013 Jan;28(1):59-65. doi: 10.1590/s0102-86502013000100010.
To assess pregnancy of rabbits submitted to bilateral ovariectomy and orthotopic allogeneic or autologous intact and sliced ovarian transplantation without a vascular pedicle and to determine the morphofunctional aspects of the transplanted ovaries.
Fifty-six female rabbits were studied. The ovaries were removed and orthotopically transplanted or replaced without vascular anastomoses: Group 1 (n=8), only laparotomy and laparorrhaphy, Group 2A (n=8) intact ovaries were transplanted on both sides, Group 2B (n=8) both ovaries were sliced and orthotopically transplanted, Group 2C (n=8), an intact ovary was transplanted on one side and a sliced ovary on the other side. In Group 3 the ovaries were reimplanted according to the procedure and subgroups described for Group 2. Three months later, the animals were paired with males for copulation. Estradiol, progesterone, FSH and LH hormone levels were assessed. A histologic study was carried out, and the number of pregnancies and litters were also determined. Chi-square test compared the number of pregnancies and litters. One-way ANOVA and the Tukey-Kramer tests compared the hormonal dosages.
Pregnancies occurred in seven (87.5%) rabbits of Group 1, in three rabbits (37.5%) of Groups 2A and 3A, in four rabbits (50%) of groups 2B, 3B and 3C, and in five (62.5%) of group 2C. Normal hormone serum levels and histology confirmed the vitality of all ovaries.
Orthotopic allogeneic and autologous ovarian transplantation without a vascular pedicle is viable in rabbits, and preserves their hormonal levels and fertile functions.
评估接受双侧卵巢切除术并进行同种异体或自体原位完整及切片卵巢移植（无血管蒂）的兔子的妊娠情况，并确定移植卵巢的形态功能方面。
研究了56只雌性兔子。切除卵巢并进行原位移植或复位，不进行血管吻合：第1组（n = 8），仅行剖腹术和腹壁缝合术；第2A组（n = 8），双侧移植完整卵巢；第2B组（n = 8），双侧卵巢切片后原位移植；第2C组（n = 8），一侧移植完整卵巢，另一侧移植切片卵巢。第3组按照第2组所述的程序和亚组进行卵巢再植入。三个月后，将动物与雄性配对进行交配。评估雌二醇、孕酮、促卵泡激素和促黄体生成素的激素水平。进行组织学研究，并确定妊娠数和产仔数。卡方检验比较妊娠数和产仔数。单因素方差分析和Tukey-Kramer检验比较激素剂量。
第1组7只（87.5%）兔子妊娠，第2A组和第3A组3只（37.5%）兔子妊娠，第2B组、第3B组和第3C组4只（50%）兔子妊娠，第2C组5只（62.5%）兔子妊娠。正常的激素血清水平和组织学证实了所有卵巢的活力。
无血管蒂的原位同种异体和自体卵巢移植在兔子中是可行的，并能保留其激素水平和生育功能。
